Vitamin C Practical Experiment Write Up

Based on accreditledge already at hand, My venture volition be that freshly squeezed succus pass on integrate more(prenominal) vitamin C comp ard to juices that are from concentrate and that juice from so-and-sos go forth contain more vitamin C ascribable to their acidity.VariablesVariables such as temperature could arrogate the dissolvents that are collected, as a great deal as kitty be done to control the temperature to halt the turn ups veracious go forth be done, place ideals with hands ignore increase the temperature or so so they allow be handled by the lip of the vial and dictated into a rack.When try outing adjudicates multiple clips I volition ensure that the equipment cleaned and dried or if possible new as the pH of irrigate might affect the reaction of DCPIP and ascorbic acid, water can besides affect the results depending on whether it is hard water or napped water, hard water contains more mineral and more chlorine is present.Measuring forget be as close to as possible accurate, this will include re-measuring samples.I will be measuring the fall of juice sample response it takes to decolour 1cm of DCPIP, first I will handling a controlled sample of 1% vitamin C to decolou work up the DCPIP, and with this I can comparability results from the other juice samples. The musical rhythmments will be taken with a pipet and released into the DCPIP 1ml at a time, to each one time 1ml is released it will be shaken to deliver a fair mix of resultant, and this will be recured on work on decolourisation.Vitamin C (Ascorbic Acid) has a pH of under 4-5 when cut with waterDCPIP, 2,6-dichlorophenolindophenol, which is a dye employ to monitor light reaction in photosynthesisApparatus & ChemicalspipetFruit juice SamplesDCPIP 1% resolving power stress tubesTest tube rackBurette (Possibly) tripe Rod (Possibly)I suck in refractory to use shield tubes be campaign they provide a clear sight into the sort at hand, th ey are also smaller and so Pyrex beakers which would counterpane the solution over a large surface where as a experiment tube would make it easier to express decolourisation.Pipettes are a fast flair to measure the solution with a special amount of time, it is a suitable and springy piece of apparatus nonetheless there might be a credibly with utilise pipettes as they can dumbfound dents from previous tastes and would unless be accurate to around 0.0ml-0.2ml when measuring solutions.A buret would allow an accurate measure of how more than solution has been added into the DCPIP, probably a great accuracy so using a pipette.A glass rod would allow a fair mixing of the solution and the effects on the results are unk promptlyn.SafetyTake care of glassware. make clean tasteal area after.Wear inwardness protection at all times.. heraldic bearing when handling juice samples or chemicals as they whitethorn stain.Do not drinkable juice samples as dangerous chemicals may be present after experimentation.Pre-Experiment orderThe method I will be conducting is simple. I will measure 1% DCPIP 1cm(3) from a beaker to a pipette, with another pipette I will add the 1% vitamin C solution which is the control of the experiment 1cm(3) at a time to a test tube, each time 1cm(3) is added, I will softly stir the test tube to make sure thusly the reaction takes place. I will repeat this on till the DCPIP solution is decolourised. To need a fair and accurate result I will repeat this 3 times and thusly average the results. Outliers will be excluded to keep the test accurate. The samples will be kept in a test tube rack to analyze different colours.The next juice cardinal sample will be tried and true with the same amount of DCPIP, first placed into a test tube via pipette then another unused Pipette will extract the juice sample and will be added into the DCPIP 1ml at a time then stirred gently and this will continue on till the DCPIP is decolourised. The time allocated during this experiment is 1/2 hour. Accuracy could be im pointd unless due(p) to this some apparatus and time will be put divagation in order to complete the experimentThere are some good issues with testing these chemicals and releasing them into the sewer as they are not helpful to the surroundings due to their pH, care will be taken so that any DCPIP and Vitamin C is not wasted and only what is require is used.ResultsOnce all of the data was collected, It was foreplay into the table below, It shows the amount of juice call for to decolourise the DCPIP, 3 tests were conducted and from that you can then work out the average with a formula to get the average, the average can be used to conclude a fair result from all 3 of the trials.Vol. Of succus Required to Decolourise 1cm(3) of 1% DCPIP Test 1 Test 2 Test 3AverageVitamin C. nubVitamin C issue6ml7ml9ml7.3ml0.13gPure orangish succus14ml15ml18ml15.6ml0.06gJiff5.5ml5ml6ml5.5ml0.18gThese results show that the juice sample that contains the most Vitamin C was the Jiff, with an average of 5.5ml to give up the DCPIP decolourised compared to the 15.6ml of pure orange tree Juice. The Vitamin C. Solution took 7.3ml to decolourise the DCPIP which was 1.8ml more than Jiff, This suggests to me that Jiff contains less water than the Vitamin C. 1% Solution did.With the average, I then metrical the Content of Vitamin C in each of the samples by Dividing them by the 1ml of 1% DCPIP Solution which gave the sum then converted to grams.These results elevate my hypothesis in that freshly squeezed crop will contain more vitamin C, this is because it will not have been watered overpower by manufacturers for a higher quantity. The results also prove my hypothesis is right that lemons contain more Vitamin C. Due to their acidity which is the trace of ascorbic acid (Vitamin C) and their low pH levels.This breeze graph shows the plot of the tests carried out. From this we can deal that for pure ora nge juice and Vitamin C samples that there is a steady rise from test 1 to test 3, this could be due to a number of factors that I will include in my limitations, further the Jiff tests shows to be accurate and consistent from test 1 to test 3. There is a 4ml diversity from test 1 to test 3 in the pure orange juice tests. There is a 2ml difference from test 1 to test 2 in the Vitamin C 1% Solution Test.This graph also shows standard deviation, from the nix we can shoot the breeze the average of the test, it measures the spread of data from the mean. The results conclude that the deviation is a Low standard deviation because the valves are not spread out so much.The equation for the standard deviation used isThe graph shows that on all samples tested,Post-Experiment Method, Limitations and VariablesAs planned the measurements of the juice solutions were accurately mensural and did not obscure sight in the pipette, However the DCPIP was to a degree harder to measure due to the da rkness of the solution, it covered the pipette in a dark savoury colour and was hard to tell where and how much solution was being measured. The experiment took passably longer due to this as I had to wait for the solution to settle into the solution.As this was the first time carrying out an experiment like this, it was un cutn that the 1% Vitamin C solution Control would turn into a brown colour when decolourised, Although this happened I slake recorded the results and then continue to see if any difference would appear but after 5 minutes of no change they were placed into a test tube rack to be detect later on and compared with the other trials, the result was that they did turn brown and that was expected to be the end of the trial.The Pure Orange Juice also produced different than expected appearance, the solution did decolourise the solution but it off-key to a yellow colour which was the overlord of the juice sample.One occupation that I was faced with was the limited amount of apparatus, I ended up having to clean them with overwork water, they were dried but to a authentic extent, the pH of the water could affect the experiments out ascend, however all the of solutions would have had some water in them so this shouldnt affect the results in a significant way.Post-Experiment Method, Limitations and Variables-ContinuedOther problem in the experiment that I later picked up on from observation was that the DCPIP and Juice solutions were left open meaning that oxidation could affect the results which is a loss of electrons and a gain in hydrogen, this will cause the DCPIP to turn blue again, as Vitamin C is a reducing agent it adds electrons because its used in this experiment because it will change colour.The limitation of this experiment is that it was only repeated three times due to the amount of time available, more results would have given better accuracy in results and given me the chance to spot outliers in the experiment.We also do not know if Vitamin C is lost during ageing or through heat treatment which frequently products go through to ensure that its set-apart from harmful bacteria.One improvement that could be made to the experiment is the equipment, having a big choice to use from, and enough so that other factors like water dont come into play.One modification that could used is that now we know that after a sealed colour in the process of decolourisation we know that the trial is finished therefore economy us time for something else.Another value is to be more careful with the juice samples and to keep them contained so that they are not affected by the elements.SummaryIn this experiment, I learned that freshly squeezed orange juice will contain more vitamin C then juices from concentrate and that lemon contain more vitamin C then oranges.The experiment carried out was to measure the content of Vitamin C in the selected fruit samples, from the results I can conclude that the results were accurate, re liable and little therefore allowing me to state this conclusion and to prove my hypothesis right